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KMID : 1020220120120030641
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Journal of Korean Society of Dental Hygiene
2012 Volume.12 No. 3 p.641 ~ p.646
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Toothbrush sterilizing effects of using microwave
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Ji Yun-Jeong
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Abstract
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Objectives: The purpose of this study is to analyze the sterilizing effects of toothbrushes by administering bacteria into toothbrushes and reproducing the antibacterial effects using a microwave oven.
Methods: The heads of four-row mid-strength toothbrushes were cut, put in a bacterial solution (3×109cells/ml) for vortexing, and sterilized with microwaves for 0, 30, and 60 seconds. They were then moved into four tubes containing DW 10 ml and suspended in a vortex mixer for two minutes to separate bacteria from them. DW 9ml was added by 1ml of bacteria for dilution of 10~106 times. It was then administered to the BHI agar plate by 0.1ml and cultured at 37℃ for 24 hours. Total number of bacteria adhered to a toothbrush was obtained by multiplying the number of colonies by the dilution factor. The experiment was done in the first, second, and third step, being repeated in a normal temperature drier (23℃) after 5, 9 and 24 hours.
Results: The results of the experiment revealed that the sterilizing effects were 95% or over. When toothbrushes were sterilized for 60 seconds, the number of colonies is about 11 after drying for 5 hours, 7 after drying for 9 hours and 2 after drying for 24 hours. The sterilizing effects reached 98% when the bacteria-administered toothbrush was sterilized for 1 minute after drying for 24 hours.
Conclusions: The results demonstrated that toothbrush sterilizing by using microwave is a suitable way to prevent cross-contamination of toothbrushes by oral bacterial infection and thus easy to use at home. However, this study suggests that toothbrush sterilizing by using microwave should be limited within two times a week because the physical properties of toothbrush might be changed.
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KEYWORD
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microwave, sterilizing effects, toothbrush
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